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DiscoverX corporation lysis buffer from the pathhunter detection kit
Lysis Buffer From The Pathhunter Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysis+buffer+from+the+pathhunter+detection+kit/lysis+buffer+from+the+pathhunter+detection+kit/pmc03591626-100-7-22
Average 90 stars, based on 1 article reviews
lysis buffer from the pathhunter detection kit - by Bioz Stars, 2026-09
90/100 stars

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Lysis:

Article Title: ?-Arrestin Recruitment and G Protein Signaling by the Atypical Human Chemokine Decoy Receptor CCX-CKR
Article Snippet: .. Cells were disrupted using 12 μl of substrate-containing lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 90 min at room temperature before measurement of β-galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer).

Article Title: β-Arrestin Recruitment and G Protein Signaling by the Atypical Human Chemokine Decoy Receptor CCX-CKR
Article Snippet: .. Cells were disrupted using 12 l of substrate-containing lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 90 min at room temperature before measurement of -galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer).

Article Title: beta-Galactosidase enzyme fragment complementation for the measurement of Wnt/beta-catenin signaling.
Article Snippet: Enzyme fragment complementation assays Cells were seeded in a 384-well CulturPlate (Perkin Elmer, Boston, MA, USA) with 0.5 104 U2OS-EFC cells or 2 104 HEK293-EFC cells in 25 l DMEM F12 containing 2% FCS, 100 U/ml penicillin and 100 g/ml streptomycin (assay medium). .. After overnight incubation at 37°C in a humidified incubator (5% CO2, 95% humidity), cells were stimulated with 2.5 l agonist in assay medium and then returned to the incubator for 3 h. Cells were disrupted using 12.5 l substratecontaining lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 1 h at room temperature before measurement of -galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer). siRNA transfection Nontargeting siRNA controls and siRNA targeting -catenin (Thermo Fisher/Dharmacon, Lafayette, CO, USA) were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions, at a final concentration of 100 nM.

Formulation:

Article Title: ?-Arrestin Recruitment and G Protein Signaling by the Atypical Human Chemokine Decoy Receptor CCX-CKR
Article Snippet: .. Cells were disrupted using 12 μl of substrate-containing lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 90 min at room temperature before measurement of β-galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer).

Article Title: β-Arrestin Recruitment and G Protein Signaling by the Atypical Human Chemokine Decoy Receptor CCX-CKR
Article Snippet: .. Cells were disrupted using 12 l of substrate-containing lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 90 min at room temperature before measurement of -galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer).

Article Title: beta-Galactosidase enzyme fragment complementation for the measurement of Wnt/beta-catenin signaling.
Article Snippet: Enzyme fragment complementation assays Cells were seeded in a 384-well CulturPlate (Perkin Elmer, Boston, MA, USA) with 0.5 104 U2OS-EFC cells or 2 104 HEK293-EFC cells in 25 l DMEM F12 containing 2% FCS, 100 U/ml penicillin and 100 g/ml streptomycin (assay medium). .. After overnight incubation at 37°C in a humidified incubator (5% CO2, 95% humidity), cells were stimulated with 2.5 l agonist in assay medium and then returned to the incubator for 3 h. Cells were disrupted using 12.5 l substratecontaining lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 1 h at room temperature before measurement of -galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer). siRNA transfection Nontargeting siRNA controls and siRNA targeting -catenin (Thermo Fisher/Dharmacon, Lafayette, CO, USA) were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions, at a final concentration of 100 nM.

Incubation:

Article Title: beta-Galactosidase enzyme fragment complementation for the measurement of Wnt/beta-catenin signaling.
Article Snippet: Enzyme fragment complementation assays Cells were seeded in a 384-well CulturPlate (Perkin Elmer, Boston, MA, USA) with 0.5 104 U2OS-EFC cells or 2 104 HEK293-EFC cells in 25 l DMEM F12 containing 2% FCS, 100 U/ml penicillin and 100 g/ml streptomycin (assay medium). .. After overnight incubation at 37°C in a humidified incubator (5% CO2, 95% humidity), cells were stimulated with 2.5 l agonist in assay medium and then returned to the incubator for 3 h. Cells were disrupted using 12.5 l substratecontaining lysis buffer from the PathHunter Detection Kit in the formulation specified by the supplier (DiscoveRx). .. Plates were incubated in the dark for 1 h at room temperature before measurement of -galactosidase activity (luminescence) on an Envision multilabel plate reader (PerkinElmer). siRNA transfection Nontargeting siRNA controls and siRNA targeting -catenin (Thermo Fisher/Dharmacon, Lafayette, CO, USA) were transfected into cells using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions, at a final concentration of 100 nM.



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DiscoverX corporation lysis buffer from the pathhunter detection kit
Lysis Buffer From The Pathhunter Detection Kit, supplied by DiscoverX corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lysis+buffer+from+the+pathhunter+detection+kit/lysis+buffer+from+the+pathhunter+detection+kit/pmc03591626-100-7-22
Average 90 stars, based on 1 article reviews
lysis buffer from the pathhunter detection kit - by Bioz Stars, 2026-09
90/100 stars
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